abstract：:We devised a microbial culture apparatus capable of maintaining sub-nanomolar H2 concentrations. This apparatus provides a method for study of interspecies hydrogen transfer by externally fulfilling the thermodynamic requirement for low H2 concentrations, thereby obviating the need for use of cocultures to study some ...

journal_title：Journal of microbiological methods

authors： Valentine DL,Reeburgh WS,Blanton DC

更新日期：2000-02-01 00:00:00

abstract：:We have developed and implemented methods of extracting morphological features from images of biofilms in order to quantify the characteristics of the inherent heterogeneity. This is a first step towards quantifying the relationship between biofilm heterogeneity and the underlying processes, such as mass-transport dyn...

journal_title：Journal of microbiological methods

authors： Yang X,Beyenal H,Harkin G,Lewandowski Z

更新日期：2000-01-01 00:00:00

abstract：:A discontinuous lactate dehydrogenase coupled assay is described for the evaluation of the pyruvate carboxylase activity (Pc, EC 6.4.1.1) in a glutamate overproducing strain of Corynebacterium glutamicum. After an initial permeabilisation period of the cells, the method consisted of the fluorometric determination of t...

journal_title：Journal of microbiological methods

authors： Uy D,Delaunay S,Engasser J,Goergen J

更新日期：1999-12-01 00:00:00

abstract：:An automated modification of the most-probable-number (MPN) technique has been developed for enumeration of phagotrophic protozoa. The method is based on detection of prey depletion in micro titre plates rather than on presence of protozoa. A transconjugant Pseudomonas fluorescens DR54 labelled with a luxAB gene casse...

journal_title：Journal of microbiological methods

authors： Ekelund F,Christensen S,Rønn R,Buhl E,Jacobsen CS

更新日期：1999-11-01 00:00:00

abstract：:Bacterial community structure was studied in a Flevo silt loam (FSL) soil microplot, as well as in 15 other soils, by using DNA extraction followed by molecular fingerprinting. Total community DNA was extracted and purified by a direct method, which yielded amplifiable DNA of high molecular weight for all soils. A var...

journal_title：Journal of microbiological methods

authors： Gelsomino A,Keijzer-Wolters AC,Cacco G,van Elsas JD

更新日期：1999-10-01 00:00:00

abstract：:We evaluated the performance of two enzyme-immunoassays (EIA) for the detection of naturally occurring, thermophilic Campylobacter spp. found in faecal samples from cattle (n = 21 and n = 26) and swine (n = 43) relative to the standard culture method, and also assuming that none of the tests was the definitive standar...

journal_title：Journal of microbiological methods

authors： Hoorfar J,Nielsen EM,Stryhn H,Andersen S

更新日期：1999-10-01 00:00:00

abstract：:A simple and reliable method to estimate paper degradation by cellulolytic bacteria is described. This method is based on the detection in the culture medium of a fluorescent whitening agent (FWA) added to white paper during the manufacturing process. Preliminary results using a Cellulomonas strain cultivated in a liq...

journal_title：Journal of microbiological methods

authors： Bichet-Hébé I,Pourcher AM,Sutra L,Comel C,Moguedet G

更新日期：1999-08-01 00:00:00

abstract：:The level of microbial resistance to heavy metals is an important issue for the microbial ecology of heavy metal-contaminated habitats. However, assays based upon growth in nutrient media will overestimate the resistance level due to metal ion interactions with inorganic and organic components. The analysis of Pb-resi...

journal_title：Journal of microbiological methods

authors： Konopka A,Zakharova T

更新日期：1999-07-01 00:00:00

abstract：:The description of microbial diversity by molecular culture-independent techniques most often involves the amplification of the 16S rRNA by PCR gene and either analysis of the diversity of amplified molecules (community fingerprinting) that allows the simultaneous study of many samples or the cloning and sequencing of...

journal_title：Journal of microbiological methods

authors： García-Martínez J,Acinas SG,Antón AI,Rodríguez-Valera F

更新日期：1999-05-01 00:00:00

abstract：:In this review, we examine numerous applications of the green fluorescent protein (GFP) marker gene in environmental microbiology research. The GFP and its variants are reviewed and applications in plant-microbe interactions, biofilms, biodegradation, bacterial-protozoan interactions, gene transfer, and biosensors are...

journal_title：Journal of microbiological methods

authors： Errampalli D,Leung K,Cassidy MB,Kostrzynska M,Blears M,Lee H,Trevors JT

更新日期：1999-04-01 00:00:00

abstract：:For flow cytometry-based detection as well as susceptibility testing and counting, staining of the bacterial cells is essential. In an attempt to develop rapid preparatory procedures for nucleic acid staining of wild type Gram positive bacteria, the uptake of fluorescent dyes in viable S. aureus, E. faecalis, and B. c...

journal_title：Journal of microbiological methods

authors： Walberg M,Gaustad P,Steen HB

更新日期：1999-03-01 00:00:00

abstract：:Microsporidia is a term used to describe a group of emerging protozoan pathogens whose environmental occurrence has only recently been documented due to lack of detection methodologies. This study evaluates and describes current methods for detection of microsporidia in water. Standard methods, for the collection and ...

journal_title：Journal of microbiological methods

authors： Dowd SE,Gerba CP,Kamper M,Pepper IL

更新日期：1999-02-01 00:00:00

abstract：:Two rapid in situ enumeration methods using fluorescent probes were used to assess the physiological activities of Klebsiella pneumoniae biofilms on stainless steel. Fluorescent dyes, 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) and rhodamine 123 (Rh 123), were chosen to perform this study. CTC is a soluble redox ...

journal_title：Journal of microbiological methods

authors： Yu FP,McFeters GA

更新日期：1994-01-01 00:00:00

abstract：:Fourier transform-infrared (FT-IR) spectroscopy has been used to rapidly and nondestructively analyze bacteria, bacteria-polymer mixtures, digester samples and microbial biofilms. Diffuse reflectance FT-IR (DRIFT) analysis of freeze-dried, powdered samples offered a means of obtaining structural information. The bac...

journal_title：Journal of microbiological methods

authors： Nichols PD,Henson JM,Guckert JB,Nivens DE,White DC

更新日期：1985-01-01 00:00:00